Objective To investigate the degree of infection of Helicobacter pylori (Hp) in the health check-up group, and to explore the relationship between degree of Hp infection and levels of serum pepsinogen ( PG). Methods 438 ca ses of health check-up people were chosen to perform the 13C urea breath test (UBT) to detect the Hp infection. The serum PG level was detected by TRFIA. Results The infection rate of Hp in health people was 47. 95%. The degree of Hp infection (III I) in female was remarkably higher than that of male. The serum levels of PGI and PGII in Hp positive group were remarkably higher than that in Hp negative group, and the ratio of PGI to PGII was remarkably lower. The rates of abnormal PG also had significant differences between two groups. The results also showed that the levels of PGI and PGII were increased as UBT value increased ,and the ratio of PGI to PGII was remarkably decreased. Conclusion The Hp infection rate and PG abnormality rate were relatively high in the health check- uppeople, and it should cause attention. The combined detection of Hp infection and PG tests could be used in the health check- up people to screen high risk group with gastric diseases.