探讨抗核抗体(ANA)和抗核抗体谱(ANAs)检测对诊断系统性红斑狼疮(SLE)患者的临床意义。用间接免疫荧光法(IIF)和免疫印迹法检测106例SLE组和30名对照组血清ANA及ANAs中的12种抗体。结果表明:SLE组ANA阳性率为86.8%，ANAs中抗ds-DNA、抗Scl-70、抗Jo-1、抗nRNP、抗Sm、抗SS-A、抗SS-B、抗Ro-52、抗CENP-B、抗AnuA、抗AHA和抗核糖体P蛋白的阳性率分别为28.3%、0.9%、0.9%、35.9%、17.0%、39.6%、18.9%、41.5%、9.4%、29.3%、31.1%和9.4%。ANA和ANAs联合检测提高了诊断的敏感性，对SLE的诊断和治疗有重要意义。

Objective To explore the clinical significance of anti-nuclear antibody (ANA) detection in the diagnosis of systemic lupus erythematosus (SLE) patients. Methods The serum ANA and anti-nuclear antibody spectrum (ANAs) of 12 kinds of antibodies in 106 SLE patients and 30 healthy control group were detected by indirect immunofluorescence assay (IIF) and western blot detection. Results The results showed that the positive rate of ANA was 86.8% in SLE patients, and positive rates of ANAs in anti-ds-DNA, anti-Scl-70、anti-Jo-1、anti-nRNP, anti-Sm, anti-SS-A, anti-SS-B, anti-Ro-52, anti- CENP-B, anti-AnuA, anti-AHA and the ribosomal P-protien were 28.3%, 0.9%, 0.9%, 35.9%, 17.0%, 39.6%, 18.9%, 41.5%, 9.4%, 29.3%, 31.1% and 9.4%, respectively. Conclusion The combined detection of ANA and ANAs could improve the sensitivity and have important significance in the diagnosis and treatment of SLE.