目的探讨高血压患者血清8-异构前列腺素F2α(8-iso-PGF2α)及其氧化应激相关酶水平改变的临床意义。方法选取193例原发性高血压患者(分为1、2、3级)，应用双抗体夹心酶联免疫吸附法(ELISA)检测患者血清8-iso-PGF2α的水平;分别应用黄嘌呤氧化酶法和硫代巴比妥法进行超氧化物歧化酶(SOD)和丙二醛(MDA)的检测;将结果与52名正常健康人的上述指标比较。结果原发性高血压1级、2级和3级患者的血清8-iso-PGF2α水平显著高于正常对照组(P＜0.05)，并与血压水平呈正相关(r=0.62，P＜0.05);原发性高血压1级、2级和3级患者的血清中SOD水平明显低于正常对照组(P＜0.01)，随着血压的升高，SOD含量有下降的趋势，呈显著负相关(r=－0.74);而MDA水平明显高于正常对照组(P＜0.01)，随血压的升高，MDA含量有上升的趋势，呈显著正相关(r=0.81)。结论血清8-iso-PGF2α、SOD、MDA水平的变化与原发性高血压的发生和发展密切相关，临床检测上述指标有利于高血压病情的评估。
刘宁
,
孙安平
,
王金松
,
刘静
,
姜楚涛
,
肖军
,
骆荣
,
金文平
,
余信之
,
汪俊军
,
周虹
. 原发性高血压患者血清8-异前列腺素F2α及氧化应激相关酶水平测定的临床意义[J]. 标记免疫分析与临床, 2013
, 20(1)
: 7
-10
.
DOI: 10.11748/bjmy.issn.1006-1703.2013.01.003
Objective To explore the changes of serum 8-iso-PGF2α,superoxide dismutase (SOD) and malondialdehyde(MDA) levels in patients with essential hypertension.Methods The content of 8-iso-PGF2α, SOD and MDA levels in 193 cases of essential hypertension and 52 controls were detected by using ELESA, xanthine oxidase and thiobarbituric acid method. Results The serum 8-iso-PGF2α levels in patients were obviously higher than those in controls (P＜0.05), the SOD levels were significantly lower than those in controls (P＜0.01) and the MDA levels were significantly higher than those in controls (P＜0.01). When the blood pressure increased, the content of SOD was decreased, and there was a significantly negative correlation (r=－0.74). But to the 8-iso-PGF2α and MDA, when the blood pressure increased the content of them was increased,There was a significantly positive correlation between the blood pressure with 8-iso-PGF2α and MDA levels respectively (r=0.62, r=0.81). Conclusion The levels of serum 8-iso-PGF2α, SOD and MDA are closely correlated to the severity of hypertension, and they may be used for evaluation of patients with hypertension.
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