摘要：目的 论证KRAS假基因对KRAS基因突变检测结果存在潜在性影响。方法 检测5个确证结直肠癌的肿瘤组织序列，并以序列比对的方式对KRASP1与KRAS基因进行同源性比较与分析。结果 KRASP1与KRAS存在高度同源性。在KRAS基因突变检测的实验中，KRASP1的存在对扩增类反应产生一定的不良影响，影响程度与检测方法的类型以及实验的设计有关。结论 KRASP1的产生与其原始序列可能是来自于同期的KRAS基因的一个转录本，并随着时间的推移逐渐差异化，但仍保留着大部分的相似性，并有进一步的分析所证实。KRASP1对KRAS基因突变检测结果存在潜在影响，如何避免同源性干扰将取决于分子诊断方法的实验设计。

Abstract：Objective To explore the potential effect of false KRAS gene on KRAS mutations detection. Methods Five confirmed sequence of colorectal cancer tumor tissues were tested and in the sequence alignment of KRASP1 with KRAS homology were compared and analyzed. Results KRASP1 had high homology with KRAS. In the detection of KRAS mutations experiments, the existence of KRASP1 had a certain adverse effect on class amplification reaction. The influence degree was related with type of test method and experiment design. Conclusion KRASP1 might have a potential impact on the detection of KRAS mutations, and how to avoid the homologous interference may depend on the molecular diagnosis method of experimental design.