摘要：目的 研究IL-8对肺癌细胞增殖和迁移的影响，初步探讨IL-8调控肺癌细胞的分子机制。方法 体外培养肺癌NCI-H157细胞，用不同浓度的IL-8刺激肺癌细胞，分别用MTT法检测IL-8对肺癌细胞的增殖作用；划痕损伤实验和Transwell小室两种方法检测肺癌细胞的迁移能力；Western blot检测Rac1和Cdc 42蛋白的表达变化。结果 IL-8促进NCI-H157细胞增殖，但随浓度增加，细胞增殖活性差异无统计学意义； 细胞划痕损伤和Transwell实验均表明IL-8可诱导NCI-H157细胞迁移，且具有浓度依赖性； Western blot结果显示，随着IL-8浓度增加， Rac1和Cdc 42的表达水平逐渐升高，以Cdc42变化最为显著。结论 IL-8可促进肺癌细胞增殖和迁移，可能与Rac1和Cdc42表达有关。

Abstract：Objective To investigate the viability and migration of lung cancer NCI-H147 cells after IL-8 stimulation, and further study the molecular mechanism. Methods The NCI-H157 cells was cultured in vivo, and it was stimulated by different concentrations of IL-8. The cell viability was detected by MTT assays and the migration ability was examined by using the wound-healing and the Transwell assays, respectively．The expression of Rac 1 and Cdc 42 were both detected by Western blot assay. Results The MTT assay showed that the viability of NCI-H147 was increased with different concentrations. Both the Wound-healing and the Transwell assays indicated the migration of NCI-H157 was enhanced dramatically with a dose-dependent manner. The Western blot assays indicated that the expression of Rac1 and Cdc42 were up-regulated with increasing the concentrations of IL-8, and the change of Cdc42 showed more apparently. Conclusion The cell viability and migration were increased by IL-8, which may be associated with the expression of Rac1 and Cdc42.