摘要：目的 探讨Notch信号通路的两种配体 DIL-4和Jagged-1与慢性丙型肝炎的关系。方法 采集慢性丙型肝炎患者及健康对照组的新鲜空腹抗凝血，分离单个核细胞，提取RNA，反转录为cDNA，应用实时定量RT-PCR技术检测DIL-4和Jagged-1。结果 慢性丙型肝炎患者组和正常对照组相比较，DIL-4和Jagged-1测定水平均明显升高，具有显著性差异，P<0.05。结论 Notch配体在慢性丙型肝炎中明显升高，且转氨酶越高，Notch配体检测水平越高，Notch配体与慢性丙型肝炎存在相关性。

Abstract：Objective To explore the relationship of two kinds of notch signaling pathway ligand DIL-4 and Jagged-1 with chronic hepatitis C. Methods The fasting of anticoagulant fresh blood of patients with chronic hepatitis C and healthy control were collected. The separation of mononuclear cells, extraction of RNA and reverse transcription for cDNA were carried out and DIL-4 and Jagged-1 were determined by real-time quantitative RT-PCR. Results The DIL-4 and Jagged-1 levels in patients with chronic hepatitis C were significantly elevated than that of normal control (P < 0.05). Conclusion The Notch ligands in chronic hepatitis C increase significantly and the higher the transaminase, the higher the Notch ligands levels.