摘要： 目的 探讨血清中CXC趋化因子家族9 (Chemokine C-X-C motif ligand 9，CXCL9)和组织金属蛋白酶抑制剂-1(Tissue inhibitor of metalloproteinases-1，TIMP-1)对脑胶质瘤恶性程度的鉴别价值。方法 使用ELISA法检测低级别和高级别脑胶质瘤组中CXCL9和TIMP-1的含量。结果 与低级别脑胶质瘤组相比较，高级别脑胶质瘤组血清CXCL9和TIMP-1含量均显著升高，且差异具有统计学差异(P<0.05)。CXCL9区分两组的受试者工作特征曲线(Receiver operator characteristic curve，ROC)下面积为0.758 (0.723，0.793)，对于高级别脑胶质瘤的诊断敏感性和特异性分别为72.7%和67.9%。TIMP-1的ROC曲线下面积为0.722 (0.694，0.751)，敏感性和特异性分别为70.5%和62.5%。利用二元Logistic回归分析评价血清CXCL9和TIMP-1联合检测的诊断价值，其曲线下面积为0.855 (0.792，0.917)，敏感性和特异性分别为80.4%和76.1%。CXCL9和TIMP-1的联合诊断与CXCL9和TIMP-1单独检测的诊断价值相比，其曲线下面积有显著提高(P=0.014，P=0.007)。结论 CXCL9和TIMP-1的联合诊断是脑胶质瘤患者鉴别诊断的一种潜在的辅助诊断方法。

Abstract: Objective To explore the diagnostic value of serum chemokine C-X-C motif ligand 9 (CXCL9) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in discriminating low-grade and high-grade glioma. Methods The serum levels of CXCL9 and TIMP-1 in the low-grade glioma group and high-grade glioma group were detected by ELISA. Results Compared to the low-grade glioma group, serum levels of CXCL9 and TIMP-1 in the high-grade gliomas showed significantly increased (P<0.05). The area under the curve (AUC) of CXCL9 for discriminating the two groups was 0.758 (0.723, 0.793), the diagnostic sensitivity and specificity were 72.7% and 67.9%. The AUC of TIMP-1 was 0.722 (0.694, 0.751), the diagnostic sensitivity and specificity were 70.5% and 62.5%. When CXCL9 and TIMP-1 were used together, the AUC was 0.855 (0.792, 0.917), the diagnostic sensitivity and specificity were 80.4% and 76.1%. Compared to CXCL9 and TIMP-1 used alone, the AUC showed significantly improved (P = 0.014, P = 0.007). Conclusion The combined detection of CXCL9 and TIMP-1 may provide a potential method in the diagnosis of glioma.