摘要：目的 探讨血小板计数假性减少的原因。方法 血小板减少病患为探究原因而进行骨髓细胞形态学检测，选取骨髓片及外周血片均不符合血小板减少的5份患者血液标本为研究对象，用EDTA-K2及3.8%枸橼酸钠分别抗凝静脉血，同时重新采集血液复查，并制作血涂片经瑞士-姬姆萨染色后显微镜下观察。结果 EDTA-K2抗凝血标本采集后立即检测和3.8%枸橼酸钠抗凝血检测的血小板计数值均在正常参考范围内；放置30min的EDTA抗凝血的血小板计数值下降,制作的血涂片中可观察到血小板聚集现象，而放置30min的3.8%枸橼酸钠抗凝血得血小板计数值在正常参考范围，制作的血涂片中未观察到血小板聚集现象。结论 EDTA依赖聚集是造成这5例患者血小板计数假性减少的主要原因。

Abstract：Objective To investigate the reasons for pseudothrornbocytopenia. Methods Venous bloods of 5 patients with pseudothrornbocytopenia were collected with dipotassium EDTA and sodium citrate respectively．Their PLTs were measured by the hematology analyzer, and blood smears were made for microzscope observation. Results PLTs of the 5 samples were in normal reference when they were measured immediatly. After 30 minutes, for EDTA-K2 anticoagulanted bloods，PLTs were significantly decreased and aggregated in blood smears. For sodium citrate anticoagulanted bloods, PLTs were in normal range and scattered in blood smears. Conclusion EDTA-K2 could accelerate the PLT aggregation and cause pseudothrornbocytopenia in some patients.