摘要:目的 探讨联合检测自身抗体、免疫球蛋白和补体在系统性红斑狼疮（SLE）诊断和病情判断中的应用价值。方法 选取SLE患者54例、其他自身免疫性疾病患者32例和正常对照30例，采用间接免疫荧光法测定抗核抗体（ANA）、免疫印迹法测定抗核提取物抗体（抗ENA抗体）、散射免疫比浊法测定免疫球蛋白和补体C3、C4。结果 SLE患者的ANA、抗dsDNA、抗Sm、抗核小体、抗U1-nRNP、抗核糖体P蛋白、抗组蛋白抗体的检测阳性率分别为87.04%、59.26%、27.78%、29.63%，37.04%、12.96%、27.78%；SLE活动组中抗dsDNA和抗核小体抗体的阳性率高于SLE非活动组，差异具有统计学意义（P<0.05）；SLE活动组IgG、IgA、IgM水平高于正常对照组，C3、C4水平低于正常对照组，差异具有统计学意义（P＜0.01）。结论 自身抗体联合免疫球蛋白和补体检测对SLE患者的临床诊断和病情判断有良好的参考价值。

Abstract: Objective To evaluate the clinical significances of combined detections of autoantibodies, immunoglobulin and complement in patients with systemic lupus erythematosus. Methods 54 SLE patients, 32 other autoimmune diseases patients and 30 healthy people were involved in the study. ANA was tested by indirect immunofluorescent method (IIF), ENA was tested by Western blot, the level of IgG, IgA, IgM, C3 and C4 were tested by turbidimetric method. Results The positive rates of ANA, anti-dsDNA, anti-Sm, AnuA, anti-U1-nRNP, ARPA and anti-Histone in SLE patients were 87.04%, 59.26%, 27.78%, 29.63%, 37.04%, 12.96% and 27.78% respectively. The positive rates of anti-dsDNA and AnuA in the SLE active group were significantly higher than the SLE inactive group (P<0.05). Immunoglobulin in the SLE active group was significantly higher than the normal control group. The C3 and C4 level was significantly lower than the normal control group (P<0.01). Conclusion The combined detection of autoantibodies, immunoglobulin and complement have great reference value for the clinical diagnosis and illness evaluation of SLE patients．