摘要：目的  了解AEG-1、β-catenin和C-myc在原发性肝细胞癌中的临床特性并进行分析。方法  以40例原发性肝癌患者术后肝癌组织作为研究对象；采集肝癌患者的临床及病理学特征，采用免疫组织化学染色及Western Blot方法测定肝癌组织中的AEG-1、β-catenin及C-myc蛋白细胞表达水平及表达载量，分析其表达情况与患者临床及病理特征的关联性。 结果  AEG-1在肝癌组织的高表达率为72.5％，以AOD值记分为0.80±0.07，Western Blot测出相对表达量为1.56±0.12；β-catenin蛋白在肝癌组织中高表达率为75.0%，以AOD值记为0.75±0.06，Western Blot测出相对表达量为1.23±0.11；C-myc的高表达率为62.5%，以AOD值记为0.69±0.06、Western Blot测出相对表达量为1.15±0.09。AEG-1、β-catenin和C-myc蛋白在肝癌组织中的表达与肿瘤TNM分期和肝癌组织病理分化程度有关（P＜0.05），差异有统计学意义；与患者的性别、年龄、肿瘤大小、HBsAg、术前AFP浓度均无明显相关（P＞0.05），差异无统计学意义。结论  AEG-1、β-catenin和C-myc有望成为评价原发性肝癌恶性程度的指导性指标，进而可以用于评估病程进展及预后； AEG-1、β-catenin和C-myc蛋白在原发性肝癌起病过程中的作用途径独立于AFP系统，为进一步研究原发性肝癌的发病机制提供了新的思路。

Abstract: Objective To investigate the expression level of AEG-1, β-catenin and C-myc in primary hepatocellular carcinoma. Methods 40 cases of primary hepatocellular carcinoma tissue were used as study samples. The clinical and pathological features of liver cancer patients were recorded. The expression level of AEG-1, β-catenin and C-myc were determined by using immunohistochemistry and Western Blot method. Results The expression rate of AEG-1, β-catenin protein and C-myc in primary hepatocellular carcinoma tissues were 72.5%, 75.0% and 62.5%; the AOD value score were 0.80 ± 0.07, 0.75 ± 0.06 and 0.69 ± 0.06; and the relative expression level was 1.56 ± 0.12, 1.23 ± 0.11 and 1.15 ± 0.09, respectively. The expression level of three protein were correlated with pathologic stage differentiation and TNM stage (P <0.05). There was no significant correlation with patient's gender, age, tumor size, HBsAg, and preoperative AFP concentration (P> 0.05). Conclusion AEG-1, β-catenin and C-myc might become guidance indexes for malignant hepatocellular carcinoma evaluation, and can be used to assess disease progression and prognosis. The role of AEG-1, β-catenin and C-myc protein in the original recurrent hepatocellular carcinoma onset process pathway may be independent from AFP system.