摘要： 目的 对3例疑难恶性疟病例进行实验室快速检测，评价快速检测方法的临床应用价值。 方法 以2014年6月至2015年5月3例因不明原因发热入院患者为研究对象，采用免疫胶体金快速检测法和显微镜检法分别检测患者血液疟原虫，采用核酸荧光染料和FCM检测白细胞计数（WBC）、红细胞计数（RBC）、血色素（Hgb）、血小板计数（PLT）等血常规指标，采用酶法检测患者C反应蛋白(CRP)、直接胆红素(DBIL)、总胆红素(TBIL)和谷丙转氨酶(ALT)等水平。结果  3例不明原因发热患者以畏寒、发热为主要症状，均出现血小板降低、CRP升高，和不同程度肝功能损害。胶体金快速检测和血涂片疟原虫显微镜检结果相同，均为恶性疟原虫感染。结论  免疫胶体金法快速检测有助于临床快速诊断疟原虫感染。

Abstract: Objective To evaluate the clinical application value of rapid detection method of plasmodium falciparum infection. Methods 3 patients with fever of unknown origin were collected in our hospital for this study. The immune colloidal gold rapid test and blood smear microscopic examination were used to detect plasmodium falciparum of patients. The nucleic acid fluorescent dyes and FCM were used to detect the white blood cell count (WBC), red blood cell count (RBC), hemoglobin (Hgb), platelet count (PLT). The levels of C reactive protein (CRP), direct bilirubin (DBIL), total bilirubin (TBIL) and alanine aminotransferase (ALT) in patients were detected by enzyme assay. Results 3 cases of patients with fever of unknown origin took the chills and fever as the main symptoms, that with the decreased platelet levels, elevated C-reactive protein (CRP), and different degree of liver function damage. The results of colloidal gold rapid detection and blood smear microscopic examination were the same, all of which were indicated plasmodium falciparum infection. Conclusion Fast detection of immune colloidal gold method is helpful for the rapid diagnosis of plasmodium falciparum infection.