目的 收集2014年秦皇岛市学龄前儿童、孕产妇、公务员体检人群乙肝五项定量检测结果，分析其乙肝流行趋势和特点，为本地区不同人群的乙肝病毒的防护和乙肝疫苗的接种做科学指导。 方法 采用时间分辨荧光免疫分析法(TRFIA )进行乙肝五项定量检测  结果  本资料乙肝阳性255例，不同人群乙肝表面抗体含量差异有统计学意义（P<0.05），各年龄段乙肝表面抗体含量差异有统计学意义（P<0.05），20～35岁表面抗体阳性率最高，50岁以上老年人最低，但高免疫应答率各组均不高。结论  建议医院利用信息化平台的优势，加强乙肝防护知识的宣传，发挥定量检测的优势，加强疫苗接种的有效性，为本地区不同人群乙型肝炎的防治提供科学指导。

Objective To explore the prevalence and characteristics of hepatitis B in different people in Qinhuangdao to provide scientific guidance on virus prevention and vaccination. Methods The total 10499 cases of preschool children, pregnant women and civil servants in health examination of Qinhuangdao in 2014 were involved in this study. The five indexes of hepatitis B were quantitatively determined by using time-resolved fluorescence immunoassay (TRFIA). Results There were 255 cases of positive hepatitis B in this study, hepatitis B surface antibody contents in different groups of people and different ages were statistically significant (P<0.05), the highest positive rates were at the age of 20~35 years old, and the lowest were among the age of 50 or older, but immune responses in each group were not high. Conclusion The results suggest to take advantage of hospital information platform to strengthen preventive knowledge of hepatitis B and enhance vaccination effectiveness, to provide scientific guidance in preventing hepatitis B for different groups of people in the region.