摘要：目的 介绍一种发现生化分析仪交叉污染及消除/减少交叉污染的方法。方法 使用Hitachi 7600型全自动生化分析仪，对所有生化检测项目（36项）进行交叉污染筛查，之后进行交叉污染的确认实验，并在确认的基础上利用增加水洗、碱洗、酸洗、调整检测顺序等办法消除或减少交叉污染。结果 发现具有交叉污染的项目39对，在采取增加水洗、碱洗和酸洗措施后改善了26对，66.7%有交叉污染的项目对被改善，剩下的13对改善效果不明显。之后在检测顺序上调整，调整后交叉污染现象均改善。 结论 交叉污染所产生的结果干扰，是生化检验中非常重要且易被忽略的问题，单纯依靠经验性观察重复检查发现问题容易造成疏漏，建议使用开放试剂的生化实验室，在仪器启用或更换试剂时，进行交叉污染筛查和确认，并采取措施，以更好地提高检测结果准确性、为临床服务。

Abstract: Objective To introduce an effective method to discover and eliminate the crossover contamination on the automatic biochemistry analyzer. Method Thirty-six clinical biochemistry items were tested and analyzed to screen the crossover contamination by Hitachi 7600 automatic biochemistry analyzer. After the confirmation on the existence of crossover contamination, water washing, acid washing, alkaline washing and adjustment of tests sequencing were applied to eliminate or reduce the crossover contamination. Results Thirty-nine pair tests with crossover contamination were found in this study. After water washing, acid washing and alkaline washing, twenty-six pair tests (66.7 %) with crossover contamination were improved. But the remaining 13 pair tests with crossover contamination were not improved. Then tests sequencing were adjusted, the remaining 13 pair tests with c crossover contamination were improved. Conclusion The crossover contamination is one of the major interferences to the accuracy of the biochemistry tests. However, this kind of contamination was easily to be neglected if only depending on the empirical observing. The screening, confirmation and corresponding measures should be applied to enhance the accuracy of results and provide better service to clinics when the clinical biochemistry lab with opening reagents begin to use the new automated biochemistry analyzer or change the reagents.