目的 建立肝活素（Hepassocin）酶联免疫吸附分析方法（ELISA）。方法 使用两株抗体，一株包被微孔板，另外一株用HRP标记。显色系统使用TMB显色。结果 测定范围在20～1000ng/mL，最低检测限在10ng/mL，回收率在93.9%～103.1%之间，批内和批间变异为9.7%和7.8% ，正常参考值为 <20ng/mL。结论 用1000份临床样本考核本试剂盒，与参比试剂盒有很好的临床符合性，与参比试剂盒具有同等的临床使用价值

Objective To develop Enzyme linked Immunoassay for Hepassocin measurement. Methods Enzyme linked Immunoassay (ELISA) for Hepassocin in serum was developed using a double antibody sandwich method, in which one strain was coated on the microplate and another strain was labeled with horseradish peroxidase (HRP). TMB color was used as Chromogenic system. Results The Hepassocin detection range was from 5 to 120ng/mL. The correlation coefficient of the standard curve was more than 0.995, and the lowest detection level was 10ng/mL. The average recovery rate was between 93.9% and 103.1%. The intra assay CV was between 9.7% and 7.8%, and the Hepassocin normal concentration in serum of healthy men was less than 20ng/mL. Conclusion The detection results with 1000 serum samples by ELISA developed in this study showed good correlation with clinical results. The ELISA had same clinical values as the control kits.