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方法研究

免疫比浊法检测梅毒螺旋体抗体的方法评价

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  • (解放军总医院生化科,北京100853)
王建柱(1982-),男,本科,检验技师,临床生化及肿瘤标志物检验工作。Tel:01066937374;E-mail:wangjz301@163.com

收稿日期: 2014-06-11

  修回日期: 2014-08-27

  网络出版日期: 2015-01-25

Method Evaluation on Serum Concentration of Anti-treponema Pallidum Antibody by Immunoturbidimetry

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  • Department of Clinical Biochemistry, PLA General Hospital, Beijing 100853, China

Received date: 2014-06-11

  Revised date: 2014-08-27

  Online published: 2015-01-25

摘要

目的 评价免疫比浊法在梅毒螺旋体抗体检测中的临床应用价值。方法 分析免疫比浊法的精密度、线性、稳定性、干扰因素、携带污染指标,并与化学发光微粒子免疫分析法(CMIA)进行阳性率比较。结果 免疫比浊法批内CV为1.64~2.22%、批间CV为3.57~3.74%;黄疸、脂血及溶血对本方法无明显干扰;阳性率均为16.8%,差异无统计学意义(X2=0,P>0.05)。结论 免疫比浊法检测结果与CMIA法检测结果相关性良好,可以满足临床诊断需要。

本文引用格式

王建柱, 周聚荷, 贾兴旺, 董振南, 田亚平 . 免疫比浊法检测梅毒螺旋体抗体的方法评价[J]. 标记免疫分析与临床, 2015 , 22(1) : 56 . DOI: 10.11748/bjmy.issn.1006-1703.2015.01.018

Abstract

Objective To evaluate clinical value of TP-Ab measurement by immunoturbidimetry. Methods The precision, linearity,stability,interfere factor and carryover in TP-Ab detection were analyzed. The positive rate of TP-Ab detection with immunoturbidimetry compared with CMIA was also analyzed. Results The within-run CV of immunoturbidimetry was 1.64~2.22%. The between-run CV was 3.57~3.74%. Icterus,lipemia and hematolysis had no significant interference on immunoturbidimetry measurement. The positive rate with both method were 16.8%, (X2=0,P>0.05).Conclusion The results of immunoturbidimetry are good correlation compared with CMIA, which is suitable for clinical application.
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