L-精氨酸对大鼠局灶性脑缺血再灌注早期炎症损伤研究

丁俊丽,贺  婕，缴克华,穆  斌，肖丽萍,银广悦

doi::10.11748/bjmy.issn.1006-1703.2014.06.030

标记免疫分析与临床 >

2014 , Vol. 21 >Issue 6: 715

DOI: https://doi.org/:10.11748/bjmy.issn.1006-1703.2014.06.030

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L-精氨酸对大鼠局灶性脑缺血再灌注早期炎症损伤研究

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丁俊丽,贺婕，缴克华,穆斌，肖丽萍,银广悦

丁俊丽（1971—），女，硕士研究生，副主任医师，从事脑与神经临床工作

收稿日期: 2014-06-05

修回日期: 2014-08-24

网络出版日期: 2014-12-26

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The Influence of L-Arginine on Early Reperfusion Injury of Focal Cerebral Ischemia in Rats

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Department of Neurology, The Central Hospital of China Petroleum Corporation, Langfang 065000, China

Received date: 2014-06-05

Revised date: 2014-08-24

Online published: 2014-12-26

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摘要

探讨一氧化氮( NO)前体L-精氨酸（L-Arg）对大鼠局灶性脑缺血再灌注早期炎症损伤的影响。方法建立大鼠局灶性脑缺血再灌注模型,设立假手术组、缺血组(ischemia/reperfusion, IS组)，L-Arg治疗组（n=10）。L-Arg治疗组于缺血2h再灌注即刻给予L–Arg，缺血组给予生理盐水。将大鼠于缺血再灌注 6 h 后断头取脑，检测脑组织中肿瘤坏死因子（TNF-α）、白细胞介素6（IL-6）和白细胞介素10（IL-10）的活性,测定脑组织含水量和脑梗死体积。结果局灶性脑缺血再灌注大鼠给予L-Arg治疗组TNFα和IL-6的表达下降，IL-10升高，脑组织含水量及脑梗死面积减少。结论 L-精氨酸可以通过抑制脑组织炎性因子TNF-α和IL-6的表达，上调IL-10的含量来发挥抗炎作用，从而对大鼠局灶性脑缺血再灌注损伤早期产生一定程度的保护作用。

关键词： 缺血再灌注; L-精氨酸; 炎性因子; 炎症损伤

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丁俊丽,贺婕，缴克华,穆斌，肖丽萍,银广悦 . L-精氨酸对大鼠局灶性脑缺血再灌注早期炎症损伤研究[J]. 标记免疫分析与临床, 2014 , 21(6) : 715 . DOI: :10.11748/bjmy.issn.1006-1703.2014.06.030

Abstract

Objectives To explore the role of L-Arginine (L-Arg) one of NO precursor on early stage of focal cerebral ischemia-reperfusion inflammatory injury in rats. Methods The rat model of focal cerebral ischemia injury were established and divided into sham operation group, ischemia reperfusion group and L-Arg treatment group (10 rats in each group). L–Arg was given immediately after 2 hours of cerebral ischemia in L-Arg treatment group, saline solution was given in ischemia group. The

rats of each group were beheaded after 6 hours focal cerebral ischemia-reperfusion. The levels of inflammatory cytokines tumor necrosis factor (TNF-α), interleukin 6 (IL-6) and interleukin 10 (IL-10) in beheaded brain of rats were detected. The brain infarction area and water content in brain tissue were also determined. Results The levels of inflammatory factor TNF-α and IL-6 in L-Arg treatment group rats were reduced and the expression of IL-10 was raised, the brain tissue water content and cerebral infarction area were also reduced. Conclusion L-Arginine play a role of anti-inflammatory by inhibiting the brain tissue inflammatory factor TNF and IL-6 and increasing the content of IL-10, which produces a degree of protection on cerebral ischemia in rats.

Key words： ; line-height: 150%; text-autospace: ">; line-height: 150%">Ischemia-reperfusion; line-height: 150%">; L-Arginine; Inflammatory factor; Inflammatory injury

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