目的 探讨针灸前后胃肠病患者血清蛋白谱表达的改变。方法 用MALDI-TOF质谱仪和血生化分析仪对7名胃肠病患者针灸前后血生化指标及血清蛋白谱进行检测。 结果 与治疗前比较，针灸可使胃肠病患者甘油三酯有降低趋势；胃肠病患者针灸治疗前后比较得到的2个蛋白差异峰，它们分别是：2625（m/z）, 2742（m/z）；接受针灸治疗前胃肠病患者组与对照组比较得到4个差异蛋白峰：2767（m/z）,2742（m/z）,2754（m/z），2568（m/z）；以统计差异最显著峰2767（m/z）（x轴）和2742（m/z）(y轴)建立坐标系进行二维分组分析，发现针灸治疗对胃肠病有明显效果，接近了正常人。结论 2742（m/z）和2767（m/z）这两个差异蛋白对揭示胃肠病的针灸治疗机理具有重要意义。

Objective To investigate serum protein spectrum expression changes for patients with gastrointestinal disease before and after acupuncture. Methods Blood biochemical indexes and serum protein spectrum for 7 patients with gastrointestinal diseases before and after acupuncture were detected by 7600DDP and MALDI-TOF mass spectrometer. Results Compared with patients with gastrointestinal disease before acupuncture treatment, the triglyceride (TG) level of patients with gastrointestinal diseases after acupuncture treatment decreased. There were 2 differential protein peaks (2625 (m/z), 2742 (m/z) between patients with gastrointestinal disease before acupuncture treatment group and after acupuncture treatment group. There were 4 differential protein peaks (2767（m/z）, 2742（m/z）, 2754（m/z），2568（m/z）) between patients with gastrointestinal disease before acupuncture treatment group and the control group; we establish two-dimensional analysis with 2767 (m/z) (x axis) and 2742 (m/z) (Y axis) and found that acupuncture treatment has obvious effect on patients with gastrointestinal disease , it is close to the normal. Conclusion 2742 (m/z) and 2767 (m/z) play an important role in acupuncture treatment mechanism of patients with gastrointestinal disease.