目的 初步建立表面健康人群血清附睾蛋白4的参考范围。 方法 体格检查与相关实验室检查无异常的表面健康人群共500名（男性200名，未绝经女性150名，绝经后女性150名）入选本研究。采用化学发光法检测入选研究对象血清附睾蛋白4水平并使用统计软件SPSS进行分析。 结果 年龄与血清HE4水平在男性呈弱正相关，在女性呈中等程度正相关。血清HE4水平在表面健康男性、未绝经女性和绝经后女性分别为46.2±10.7 pmol/L，45.9±10.3 pmol/L和51.7±11.7 pmol/L。 结论 血清HE4水平与性别和绝经与否有关，单侧95%参考范围在男性、未绝经女性和绝经后女性分别为<63.7 pmol/L，<62.8 pmol/L和<70.9 pmol/L。

Objective To establish the distribution of serum human epididymis protein 4 by gender and menopausal status in apparently healthy individuals. Methods The chemiluminecence immunoassay technology was used to determine the levels of serum HE4 in 500 apparently healthy individuals which included 200 males, 150 premenopausal females and 150 postmenopausal females. Results The scatter plots showed a weak correlation between age and HE4 level in male (Pearson coefficient 0.228) and a moderate correlation in female (Pearson coefficient 0.472). The serum HE4 levels were 46.2±10.7 pmol/L in male, 45.9±10.3 pmol/L in premenopausal female and 51.7±11.7 pmol/L in postmenopausal female respectively. Conclusion Gender and menopausal status influence on serum HE4 level was demonstrated. The reference range of serum HE4 in Beijing population are <63.7 pmol/L for male, <62.8 pmol/L for premenopausal female and <70.9 pmol/L for postmenopausal female.