目的 探讨酶联免疫吸附试验非平衡法对乙型肝炎e抗体和核心抗体检测结果的影响。 方法 收集电化学发光免疫分析法（ECLIA）检测抗-HBe阳性标本44例和抗-HBc阳性标本57例，再用酶联免疫吸附试验法(ELISA)对阳性标本进行检测，样本加入反应孔中分别于0min、30min 后加入酶标志物，余下步骤均严格按照说明书操作。 结果对ECLIA检测抗-HBe阳性标本44例、抗-HBc阳性标本57例进行ELISA复检后，平衡法抗-HBe的漏检率为52.3%，抗-HBc漏检率为40.4%；非平衡法抗-HBe的漏检率为34.1%，抗-HBc漏检率为24.6%。 结论 竞争法检测抗-HBe和抗-HBc时应适当延迟加酶的时间，以降低漏检率。

Objective To explore the influence of enzyme-linked immunosorbent assay method of non-equilibrium on the detection of e antibody and core antibody in hepatitis B. Methods 44 cases of anti-HBe positive and 57 cases of anti-HBc positive specimens determined by electrochemical luminescence immunoassay were analyzed by enzyme linked immunosorbent again. The enzyme marker were added into reaction holes at 0 min, 30 min after samples add respectively, the remaining steps were followed in strict accordance with the manual operation. Results The miss positive detection rate of anti-HBe and anti-HBc analyzed by equilibrium method were 52.3% and 40.4% respectively. The miss positive detection rate of anti-HBe and anti-HBc analyzed by non equilibrium method were 34.1% and 24.6% respectively. Conclusion It would be better to prolong the adding time of enzyme in the detection of anti-HBe and anti-HBc by enzyme-linked immunosorbent assay in order to increase the detection sensitivity.