目的 建立骨碱性磷酸酶（BAP）酶联免疫吸附分析方法（ELISA）。方法 使用两株抗体，一株包被微孔板，另外一株用HRP标记。显色系统使用TMB显色。结果 测定范围在5～120ng/mL，最低检测限在1.5ng/mL，回收率在96.4%～102%之间，批内和批间变异分别为5.6%和7.9% ，正常参考值为 <10ng/mL，用502份临床样本考核本试剂盒，与参比试剂盒有很好的临床符合性，与参比试剂盒具有同等的临床使用价值。

Objective To establish an enzyme linked immunoassay (ELISA) for human bone alkaline phosphatase (BAP). Methods A double antibody sandwich method was used to develop the ELISA method, in which one strain was coated on the microplate and another strain was labeled with horseradish peroxidase (HPR). TMB color was used for chromogenic system. Results The BAP detection range was from 5 to 120ng/ml, the correlation coefficient of the standard curve was more than 0.995, and the lowest detection level was 1.5ng/ml. The average recovery rate was between 96.4% and 102%, the intraassay CV was between 5.6% and 7.9%, and the BAP normal concentration in serum of healthy men was less than 10ng/ml. 502 serum samples were tested by this method, and the results of the ELISA corresponded to those of the control kits. Conclusion the ELISA developed in this study might be suitable for bone alkaline phosphatase analysis.