目的 评价导流杂交和实时荧光定量（real-time）PCR对宫颈癌患者HPV感染检测的临床意义。方法 采用导流杂交和real-time PCR分别对190例患者进行HPV感染检测，并对检测结果进行分析。结果 在190例检测标本中，导流杂交法检测出113例HPV阳性，阳性检出率为59.5%，筛查宫颈癌的敏感度为58.1％，特异度为39.2％，阳性预测值为47.8％，阴性预测值为49.4％。real-time PCR检测出106例阳性，阳性检出率为55.8%，筛查宫颈癌的敏感度为54.8％，特异度为43.3％，阳性预测值为48.1％，阴性预测值为50.0％。导流杂交和real-time PCR检出的HPV阳性率差异无统计学意义，而且一致性检验显示两种方法具有较高的一致性(符合率为87.9％)。结论 本研究结果表明，导流杂交和real-time PCR方法检测HPV未表现出较大的差异，在宫颈癌筛查中可以将两种方法结合使用，以提高检测的准确性，降低漏诊率。

Objective To evaluate the clinic value of flow through hybridization and real-time PCR in detecting HPV of cervical carcinoma. Methods 190 cases of HPV of cervical carcinoma were detected by using flow through hybridization and real-time PCR, respectively. Results There were 113 positive cases (59.5%) detected with flow through hybridization, and the sensitivity, specificity, positive-predictive value and negative-predictive value in detecting HPV for cervical carcinoma were 58.1％, 39.2％, 47.8％ and 49.4％ respectively. There were 106 positive cases (55.8%) detected with real-time PCR, and the sensitivity, specificity, positive-predictive value and negative-predictive value in detecting HPV for cervical carcinoma were 54.8％, 43.3％, 48.1％and 50.0％ respectively. There was a concordance rate of 87.9% in the detection of HPV between two methods. Conclusion The results of this study suggest that there are no significant differences in the detection of HPV between flow through hybridization and real-time PCR. The detective rate may be improved in screening cervical carcinoma by combination of the two methods.