目的 了解唐山地区病毒性腹泻的病原学，常见病毒在不同年龄人群中所引发的并发症和不同年龄住院病人给家庭带来的经济负担。方法 唐山市区5所大型综合医院联合，对住院的疑似病人进行研究。轮状病毒、腺病毒、星状病毒和诺如病毒采用ELISA方法每周测定一次，检测各类病毒的比例。阳性标本各随机抽取30份，送到北京中同蓝博临床检验所用实时荧光定量PCR技术进行基因分型。采用医院的医院信息系统（hospital information system，HIS）系统查找临床症状、各项检查，发现其在不同年龄人群引发的并发症，采用医院的HIS系统查找患者的住院费用，并自制调查表了解不同年龄患者给家庭所带来的经济负担。结果 869份标本中轮状病毒阳性的标本为346例，腺病毒阳性的为99例，诺如病毒阳性的为155例，星状病毒阳性的为65例。轮状病毒基因型以G3P[8]为主, 诺如病毒基因型以GII型为主，星状病毒以I型为主，腺病毒以41型为主。除了引起肠炎以外，轮状病毒还可引起心肌、肝脏损伤，严重的可引发惊厥、脑炎。腺病毒易引发肺炎，严重者可合并急性呼吸窘迫综合症、呼衰。诺如病毒和星状病毒几乎不引起其他并发症。疾病负担儿童高于其它人群。结论 唐山地区病毒性腹泻的分子生物学调查与其他各地区一致，以轮状病毒为主，基因型以G3P[8]为主， 引起的并发症最多。儿童的疾病负担最重。

Objective To investigate the etiology of viral diarrhea among Tangshang region, the complication cased by common virus in different age group and the economic burden for the family at different age hospitalized patients. Methods The study was conducted in suspected patients in 5 large comprehensive hospitals of Tangshan. All the viruses including Rotavirus, Norovirus, Astrovirus and Adenovirus were detected once a week by ELISA. 30 positive specimens were random sent to Beijing CIC Clinical research institute for determination of genotype by RT-PCR. Results Among 869 samples, the positive rotavirus accounted for 346, norovirus accounted for 155, astrovirus accounted for 65, and adenovirus accounted for 99. The genotype of rotavirus, norovirus, astrovirus and adenovirus were G3P [8], GII, AstvI and Hadvs41 respectively. The rotavirus could cause myocardium, liver damage, seizures and encephalitis. Adenovirus caused pneumonia and severe cases could cause complicating acute respiratory distress syndrome, and failure. Norovirus and astrovirus almost did not cause other complications. The disease burden in children was much higher than that of in other people. Conclusion The viral diarrhea molecular biology in Tang shan area is in line with other regions, The rotavirus with the genotype G3P [8] is dominated and cause most complication and it brings the heaviest burden in children.