摘要：目的 建立一种可用全自动生化分析仪定量检测血浆中髓过氧化物酶浓度的胶乳增强免疫比浊法，用于心脑血管疾病的预测和辅助急性冠脉综合症诊断。 方法 针对三个厂家的7株单克隆抗体，采用每株抗体单独与胶乳颗粒交联，逐一配对的方法，筛选出最佳的两株单克隆抗体组合。进一步对胶乳颗粒的粒选、反应体系进行筛选和优化。评价本方法的重复性，线性，灵敏度，抗干扰能力和回收率，并进行了大量的样本检测。结果 筛选到两株最适单克隆抗体，使单克隆抗体与胶乳颗粒通过化学交联的模式连接，建立了用于髓过氧化物酶检测的胶乳增强免疫比浊法。该方法的灵敏度：LoB=4ng/mL，LoD=6ng/mL，LoQ=12ng/mL；批内变异系数（CV）：2.85%；批间变异系数（CV）:3.45%；平均回收率：100.0%±1.2%；参考值范围：54～266ng/ml。结论 本法可用全自动生化分析仪定量检测髓过氧化物酶，具有较高的灵敏度和稳定性，操作简单，在心脑血管疾病的预测和辅助诊断中有很好的应用前景。

Abstract：Objective To establish a quantitative latex enhanced turbidimetric immunoassay for determination of plasma myeloperoxidase (MPO) concentration. Methods 7 anti-MPO monoclonal antibody were selected from 3 different companies, the optimum double-antibody combination was picked out by the method of each monoclonal antibody crosslink latex particle and one by one match. The suitable latex and reaction concentration were analyzed. Consequently, the repeatability, linearity, sensitivity, specificity and recovery rate of the assay were evaluated by testing a large number of samples. Results Optimum double-antibody was selected successfully. The quantitative MPO Latex enhanced turbidimetric immunoassay was set up by monoclonal antibody and the latex particles crosslink by chemical method. The results showed that the sensitivity: LoB=4ng/ml，LoD=6ng/ml，LoQ=12ng/ml, the within-run and interday- run coefficients of variation were:2.85% and 3.45%, and the mean recovery rate was 100.0%±1.2%, reference range was 54-266ng/ml. Conclusion An automated quantitative Latex enhanced turbidimetric immunoassay for determination of MPO is established. This method with high sensitivity, good stability, simply operation shows a better application to predict and diagnose cardiovascular diseases.