摘要: 自身免疫性疾病(autoimmune diseases，AID)确切的发病机制目前尚不清楚，且临床症状和体征不典型，对其诊断和鉴别诊断较复杂，但由于该类疾病都存在一些特异性的自身抗体，所以对自身免疫性疾病的诊断不仅依赖于临床表现，而且很大程度上取决于免疫学实验诊断，特别是实验室的抗核抗体(antinuclear antibody，ANA) 检测[1]。通过对ANA 类型及其滴度的检测，能为自身免疫性疾病的诊断、分型、治疗和预后判断提供重要的依据，本文就ANA和ANA谱的检测方法及其临床应用做一综述。

Abstract Autoimmune diseases (AID) are characteried by unknown pathogenesis, as well as atypical clinical symptoms, so the diagnosis and differential diagnosis for AID are more complicated. Since all kind of autoimmune diseases have highly specific antibody, the diagnosis not only depends on the clinical symptoms but also largely on the immunological experiments, especially the detection of antinuclear antibody. The identification and titer testing of ANA can provide great clinic value to the diagnosis, classification, treatment and post-prediction judge for AID. The paper will review the clinical application of ANA and ANA profile.