摘要：目的 通过对ANA核型分布及核型与特异性抗体相关性分析，为临床对ANA和ANA谱检测结果判读提供参考。方法 用间接免疫荧光法检测ANA，免疫印迹法检测ANA谱，对449例ANA或ANA谱阳性的结果进行核型和特异性抗体分析。结果 ANA核型分布以核颗粒型为主，占78.2%；核型与特异性抗体有一定的相关性，但同一种自身抗体可出现不同的荧光核型。结论 ANA和ANA谱同时检测能提高AID的检出率，有助于准确的判断特异性抗体，对AID的诊断、病情监测、预后判断有十分重要的作用。

Abstract: Objective To explore the karyotype distribution of antinuclear antibodies (ANA) and its correlation with specific antibody, to provide the reference for interpretation of clinical test results of ANA and ANA spectrum. Methods The antinuclear antibodies and antinuclear antibodies spectrum of 449 cases were detected by indirect immunofluorescence and immunoblotting respectively, and the karyotype distribution of antinuclear antibodies and its correlation with specific antibody were analyzed. Results The majority of karyotype distribution of ANA was nuclear granular type, accounting for 78.2%. The Karyotype of ANA had certain correlation with specific antibody, but the same kinds of autoantibodies could appear different fluorescence karyotype. Conclusion Simultaneous detection on ANA and ANA spectrum could improve the detection rate of AID. The detection of ANA has a high value in the diagnosis, monitoring and prognosis judgment of AID.