Abstract: Objective To explore the application value of the 13 kinds of monoclonal antibody for leukemia immunophenotyping. Methods The immunophenotye of 99 cases of acute leukemia were determined by flow cytometry (FCM) with 13 monoclonal antibodies, and compared with morphological classification results. Results The results showed that 59 cases of acute marrow leukemia (AML), 27 cases of acute lymphocyte leukemia (ALL),5 cases of mixed acute leukemia (MAL) and 2 cases of acute undifferentiated leukemia (AUL) were differentiated by using these monoclonal antibodies. The diagnosis rate was 93.9% (93/99). The expression of lineage antigens in AML was CD13> CD33> CD14, CD7> CD2, CD19> CD10> CD5> CD2, most express CD13 and CD33；the expression of lineage antigens in ALL was CD19> CD13> CD10> CD20> CD33> CD5> CD7>CD2, CD14, most express CD10, CD13, CD19 and CD20. The coincidence rate with morphology results was 90.5% for AML and 76.5% for ALL. Among these, one M0 was B lymphocyte leukemia and marrow leukemia mixed, one M1 was T lymphocyte leukemia and marrow leukemia mixed, one M2a was T lymphocyte leukemia and marrow leukemia mixed, one L1 was B lymphocyte leukemia and marrow leukemia mixed, one L2 was T and B lymphocyte leukemia mixed, one L2 was Ly+-AML, two L2 was AUL, one lymphocyte-monocyte mixing expressing CD7, CD13 and CD14. Conclusion Each Immunophenotype of AL has own character, and might need multifold monoclonal antibody to analysis. Although AL has the same morphology type, its Immunophenotype might not same, and vice versa. The combination of monoclonal antibody which our laboratory choosed are sufficient to analysis Immunophenotype of AL on the whole, and also can diagnose some mixed acute leukemia and acute undifferentiated leukemia.
