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方法研究

呼吸道合胞病毒荧光纳米颗粒快速检测试纸条检测效能评价

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  • 1广东医学院附属医院,广东湛江524001;
    2.广东粤海饲料集团有限公司,广东湛江524017
潘庆军(1978—),男,博士,讲师,主要从事创新型体外诊断试剂研究。E-mail: stilwapan@gmail.com; Tel: 0759-2387164

收稿日期: 2013-04-23

  修回日期: 2013-07-22

  网络出版日期: 2013-10-14

基金资助

国家自然科学基金(81202346);广东省自然科学基金(S2012040006216);广东省卫生厅(B2012284);湛江市科技攻 关项目(2012C3101028)

Evaluation of Performance of Respiratory Syncytial Virus Fluorescent Nanoparticles Rapid Detection Test Strip

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  • Guangdong Medical University Afiliated Zhanjiang Hospital, Zhanjiang 534000 , China

Received date: 2013-04-23

  Revised date: 2013-07-22

  Online published: 2013-10-14

摘要

目的 评估呼吸道合胞病毒(RSV)荧光纳米颗粒快速检测试纸条的灵敏度和特异性等检测效能。方法 以培养的 RSV和其他呼吸道病原体以及280例具有上呼吸道感染症状的患者作为标本来源。分别anjiang采用RSV荧光纳米颗粒快速检测试纸条和胶体金快速检测试纸条检测同一份标本,分析两种检测试纸条的检测效能。结果 荧光纳米颗粒快速检测试纸条的灵敏度高于胶体金快速检测试纸条;对甲型流感病毒、乙型流感病毒、副流感病毒、呼吸道腺病毒和肺炎支 原体无特异性反应。结论 RSV荧光纳米颗粒快速检测试纸条在检测性能上高于胶体金快速检测试纸条。

本文引用格式

潘庆军,朱学芝 . 呼吸道合胞病毒荧光纳米颗粒快速检测试纸条检测效能评价[J]. 标记免疫分析与临床, 2013 , 20(4) : 253 -256 . DOI: 10.11748/bjmy. issn. 1006 -1703.2013.04.018

Abstract

Objective To evaluate the sensitivity and specificity of respiratory tract syncytial virus ( RSV) fluorescent nanoparticles rapid detection test strip and other detection performance. Methods The specimen including RSV , other respiratory pathogens and 280 cases of patients with symptoms of upper respiratory tract infection were used as samples. The RSV fluorescent nanoparticles rapid detection test strip and colloidal gold rapid detection test strips were used to detect the same samples. Results The sensitivity of fluorescent nanoparticles rapid detection test strip were higher than colloidal gold rapid test strip, and there were no specific reaction with influenza A virus, influenza B virus, parainfluenza virus, respiratory adenovirus and mycoplasma pneumoniae. Conclusion The detection performance of RSV fluorescent nanoparticles rapid detection test strips are better than colloidal gold rapid detection test strip.
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