目的 比较化学发光法（CLIA)和酶联免疫法（EIA)测抗-HCV对诊断HCV感染的检出率，分析CLIA测定抗-HCV 作为HCV感染初筛试验的重要临床意义。方法 分别用CLIA、EIA方法检测所有临床标本中的抗-HCV，选取抗-HCV初 筛阳性样本进行HCV RNA确认试验。结果 6461例样本中，EIA和CLIA测抗-HCV阳性率分别为2. 86%和3.17% ;确 认试验中，CLIA法抗-HCV阳性与HCV RNA的符合率为97.1 %，显著高于EIA组（91. 9% )。EIA法测抗-HCV, S: CO> 5.0以上，与HCV RNA检测符合率可达100% ;弱阳性样本，与HCV RNA符合率为69. 7% ;CLIA法测抗-HCV,S:CO > 2.0以上，与HCV RNA检测符合率可达100% ;弱阳性样本，与HCV RNA符合率为92.9%。有31例样本CLIA法抗-HCV 和HCV RNA阳性，而EIA法抗-HCV阴性;有4例样本EIA法检测为阳性，而CLIA法和PCR确认试验均为阴性。结论 CLIA法测抗-HCV作为HCV感染初筛实验,与传统EIA相比,特异性和阳性预测值更高,有效降低了假阳性率，有利于临 床医生对HCV感染患者的早期诊断和治疗。

Objective To compare the detection rate of Anti-hepatitis C virus (anti-HCV) with chemilumines- cence immunoassay (CLIA) and enzyme immunoassay (EIA)，and explore the clinical significance of CLIA in diagnosis of HCV infection. Methods The anti- HCV in 6461 patient samples were detected by CLIA and EIA respectively，and anti - HCV positive samples were confirmed with HCV- RNA test. Results The positive rate of anti-HCV in 6461 patient samples determined by CLIA and EIA were 3. 17% and 2. 86%，respectively. The positive coincidence ratio of CLIA detection confirmed by HCV RNA assay (97. 1% ) was obviously higher than that of EIA (91. 9% ) . Among EIA detection samples，while S ： CO value was more than 5. 0，the positive coincidence ratio confirmed by HCV RNA was 100% . In weak positive samples，the positive coincidence ratio was only 69. 7%. Among CLIA detection samples，while S ： CO value was more than 2. 0，the positive coincidence ratio confirmed by HCV RNA was 100% . In weak positive samples，the positive coincidence ratio was 92. 9% . 31 anti- HCV positive samples detected by CLIA were negative detected by EIA. 4 anti - HCV positive samples detected by EIA were negative detected by CLIA. Conclusion CLIA provides higher specificity and positive predictive value than EIA，and reduces false- positive results. It is effective laboratory procedures for early diagnosis and treatment monitor of hepatitis C vims infection with CLIA.