Objective To evaluate the Vitamin D nutritional status in male patient with type 2 diabetes (T2DM) and its effects on bone markers. Methods 258 cases of male patients with T2DM were recruited, their serum levels of 25-hydroxyvitamin D[25（OH）D], tPINP and β-CTX were measured by Roche E170 automatic electrochemiluminescence immunoassay analyzer. According to 25（OH）D levels, patients were divided into 4 groups: severe deficiency (<25 nmol/L) , deficiency (≥25 nmol/L and <50 nmol/L) , insufficiency (≥50 nmol/L and <75 nmol/L) and sufficiency (≥75 nmol/L) groups. Results The total 25（OH）D level in T2DM patients was 33.80±18.43 nmol/L，which indicated vitamin D deficiency．The participants were divided into 4 age groups, middle-aged group (35-49 year old), presenium group (50-59 year old), gerontism group 1 (60-69 year old), gerontism group 2 (>70 year old). As the increase of age, the concentration of 25（OH）D decreased gradually. The 25 (OH) D levels in gerontism group 2 was significantly lower than that of middle-aged group, but bone markers had no significant difference among various age groups. The proportion of vitamin D in severe dificiency，defiency，insufficiency and sufficiency groups were 36.43%（94/258）, 48.45%（125/258）, 11.63%（30/258） and 3.49%（9/258）respectively. The level of β-CTX in severe deficiency group was apparently higher than other groups (P<0.05). tPINP had no difference between various nutritional status groups. The level of serum 25（OH）D was negatively correlated with serum β-CTX (r=-0.197，P=0.001), however 25（OH）D had no relevance with tPINP（r=-0.046，P=0.464）. Conclusion Vitamin D severe deficiency and insufficiency are highly prevalent among male patient with type 2 diabetes.There is no evidence shows that the serum 25（OH）D severe deficiency is associated with bone formation, while the bone absorption increases.