Abstract：Objective To discover the biomarkers of serum of systemic lupus erythematous by proteomic methods. Methods Peripheral blood was obtained from 9 SLE patients and 9 healthy controls and then mononuclear cells were isolated and the total protein was extracted by one-step method. Two-dimensional gel electrophoresis was performed and then stained with silver. Protein maps were analyzed and differentially expressed protein spots were detected using Image Master 2D Platinum5.0 software. Results Match rates of 84.5％ and 82.5％ was obtained from gels from controls and patients respectively. 648 spots were detected from control gels and 639 from patient gels. 8 protein pots were up regulated and 6 were down—regulated in SLE patients. 5 proteins were identified by MS analysis, some of which had previously been shown to play a potential role in the pathogenesis of SLE. Conclusion There are significant changes in the protein expression of peripheral blood mononuclear cells in systemic lupus erythematosus patients. This study could be used as a preliminary work for better understanding of the pathogenesis and immune regulation pathways of SLE from integrated lymphocyte protein profile perspective.