目的 探讨“小三阳”患者HBV-DNA含量与前S1抗原的关系。方法 收集乙型肝炎“小三阳”患者血清152例，用 ELISA方法检测前S1抗原，用实时荧光定量PCR(FQ-PCR)方法检测HBV-DNA。结果 前S1与HBV-DNA同时阳性者 74例，二者同时阴性者34例，符合率为71.1%。前S1阳性、HBV-DNA阴性者为30例，HBV-DNA阳性而前S1阴性者为 14例。即“小三阳”患者中前S1的检出率为68.4%，HBV-DNA的检出率为57. 9%，并且拷贝数高低有差别。结论 “小 三阳”患者体内也存在着不同程度的病毒复制。前S1的检测不能代替HBV-DNA，只能作为其补充指标。对于“小三阳” 患者，同时检测HBV-DNA定量有助于判断病毒在体内是否复制。

Objective To investigate the relationship between HBV-DNA with pre S1 antigen in patients with HBsAg，Anti-HBe and Anti-HBc positive hepatitis B. Methods The serum express of pre S1 antigen and HBV- DNA in 152 patients with HBsAg，Anti-HBe and Anti-HBc positive hepatitis B were detected by ELISA and PCR(FQ-PCR) respectively. Results 74 cases of samples were both pre S1 and HBV-DNA positive，while 34 cases were found Pre S1 and HBV-DNA negative. The positive coincidence rate between Pre S1 and HBV-DNA was 71. 1%. There were 30 cases with pre S1 positive and HBV-DNA negative，while 14 cases had HBV-DNA positive and pre S1 negative. The detection rate of pre S1 and HBV-DNA in patients with HBsAg，Anti-HBe and Anti-HBc positive hepatitis B were 68. 4% and 57. 9%，respectively. Conclusion There are virus replications of different degrees in patients with HBsAg，Anti-HBe and Anti-HBc positive hepatitis B. The detection of Pre S1 can not replace HBV-DNA detection，and it could be only used as a supplementary index. The HBV- DNA quantitative detection could facilitate to judge whether virus in-vivo replicate or not.